Studies on the primary sequence requirements for PKC-alpha, -beta 1 and -gamma peptide substrates. |
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| Authors: | R M Marais O Nguyen J R Woodgett P J Parker |
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| Institution: | Ludwig Institute for Cancer Research, London, UK. |
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| Abstract: | The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguishable in their phosphorylation of these peptides, PKC-gamma shows a distinct specificity profile for these synthetic substrates. |
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