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| 改组猪IL-2和重组IL-6C基因增强猪口蹄疫疫苗的免疫应答 | |
| 引用本文： | 郑敏,陈文龙,杨肖,程驰,李栋,王泽洲,章华兵,李悦,李江凌,胡晓云,高荣.改组猪IL-2和重组IL-6C基因增强猪口蹄疫疫苗的免疫应答[J].四川动物,2009,28(5). |
| 作者姓名： | 郑敏 陈文龙 杨肖 程驰 李栋 王泽洲 章华兵 李悦 李江凌 胡晓云 高荣 |
| 作者单位： | 1. 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都,610064 2. 四川理工学院生物工程系 3. 四川省畜牧科学研究院 4. 四川大学校医院检验科 |
| 基金项目： | 国家自然科学基金，四川省科技支撑项目 |
| 摘 要： | 以离子交联法制备壳聚糖纳米颗粒,包裹重组改组的猪IL-2基因表达质粒(VRIL2S)、重组IL-6和CpG(VPIL6C)质粒(CNP-VRIL2S-VPIL6C-CNP), 按0.5 mg/头肌肉注射壳聚糖包装质粒接种21日龄健康三元杂交猪,同时肌肉注射免疫灭活口蹄疫疫苗;在免疫后7、14、28、42和56 d,采血检测实验猪的免疫球蛋白、特异抗体和免疫细胞的动态变化.结果发现:接种后56 d,改组猪IL-2和重组IL-6C质粒接种猪血液中的特异性抗口蹄疫抗体、IgG、IgA和IgM含量均较对照空白疫苗免疫猪显著升高(P<0.05),其IL-2、IL-6水平和淋巴细胞、单核细胞的数量也相应较灭活疫苗对照组明显增加(P<0.05).这些证明猪IL-2和IL-6基因与CpG有良好的免疫协同增强效应,能有效地增强疫苗的特异体液和细胞免疫应答反应,可作为安全高效的新型免疫基因分子佐剂,促进猪对口蹄疫疫苗的免疫防御抗病力,抵御口蹄疫的感染. |
| 关 键 词： | 猪 IL-2基因 IL-6基因 免疫 口蹄疫疫苗 |
Enhanced Immunity of Pigs to Foot and Mouth Disease Vaccine by Inoculation with Shuffled Pig IL-2 Gene and Recombinant IL-6C Plasmids |
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| ZHENG Min,CHEN Wen-long,YANG Xiao,CHENG Chi,LI Dong,WANG Ze-zhou,ZHANG Hua-bing,LI Yue,LI Jiang-lin,HU Xiao-yun,GAO Rong.Enhanced Immunity of Pigs to Foot and Mouth Disease Vaccine by Inoculation with Shuffled Pig IL-2 Gene and Recombinant IL-6C Plasmids[J].Sichuan Journal of Zoology,2009,28(5). | |
| Authors: | ZHENG Min CHEN Wen-long YANG Xiao CHENG Chi LI Dong WANG Ze-zhou ZHANG Hua-bing LI Yue LI Jiang-lin HU Xiao-yun GAO Rong |
| Institution: | 1.Key Laboratory for Bio-Resource and Eco-Environment of Ministry Education;College of Life Sciences;Sichuan University;Chengdu 610064;China;2. Bioengineering Department;Sichuan University of Science and Engineering;3. Sichuan Provincial Academy of Animal Science;4. Clinical Laboratory of Sichuan University Hospital |
| Abstract: | The recombinant shuffled pig IL-2 gene and IL-6 gene plasmids with CpG motifs inserted were entrapped with chitosan nanoparticles(CNP) prepared by the ionic cross linkage method(CNP-VRIL2S-VRIL6C) ,and were then injected intramuscularly to inoculate hybrid swine which were co-immunized with the inactivated foot and mouth disease vaccine(FMDV) . Blood was collected from the pigs on 7,14,28,42,and 56 days post vaccination to detect immunoglobulin changes,specific antibodies,IL-2,IL-4,IL-6,and immune cells. Th... |
| Keywords: | CpG |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |