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n-Amyl alcohol as a substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacteria
Authors:Shunsaku Ueda  Seiji Matsumoto  Aya Takagi  Tsuneo Yamane
Affiliation:Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agricultural Sciences, Nagoya University, Nagoya, Japan
Abstract:Abstract n -Amyl alcohol was examined as a source for the synthesis of the 3-hydroxyvalerate (3HV) unit of the biopolyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)), by Alcaligenes sp., Pseudomonas sp. and several methylotrophic bacteria. A. eutrophus and Ps. lemoignei synthesized P(3HB-co-3HV) from glucose and n -amyl alcohol under nitrogen-deficient conditions. Many of methylotrophic bacteria grown on methanol synthesized the copolyester from methanol and n -amyl alcohol under nitrogen-deficient conditions. The content and composition of the polyester varied from strain to strain. Paracoccus denitrificans differed from all others in having a higher content of 3-hydroxyvalerate units in the copolyester synthesized.
Keywords:n-Amyl alcohol    Copolyester    Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)    Methyltroph
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