Effects of Selected Assay Parameters on Measurement of Lignocellulose Mineralization with a Radiolabeled Substrate

Varying the amount of labeled substrate or the amount of leaf material resulted in significant nonlinear changes in lignocellulose mineralization, as measured with natural ¹⁴C]lignin-labeled lignocellulose. The use of periodic rather than continuous aeration was found not to have significant effects on measured mineralization.