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| 嗜酸乳杆菌黏附鸡胚肠黏膜上皮细胞能力的研究 | |
| 引用本文： | 王术德,曲练达,魏萍. 嗜酸乳杆菌黏附鸡胚肠黏膜上皮细胞能力的研究[J]. 中国微生态学杂志, 2007, 19(2): 145-147,149 |
| 作者姓名： | 王术德 曲练达 魏萍 |
| 作者单位： | 1. 辽宁医学院动物医学院,微生物教研室,辽宁,锦州,121001 2. 辽宁医学院动物医学院,预防兽医学教研室,辽宁,锦州,121001 3. 东北农业大学,预防兽医学教研室,黑龙江,哈尔滨,150030 |
| 摘 要： | 目的建立肠黏膜上皮细胞模型和测定嗜酸乳杆菌的黏附能力。方法将嗜酸乳杆菌用胃蛋白酶和HCl-H2O低pH以及反复冻融、灭活等方法处理后测定其黏附能力。结果成功地建立了鸡胚肠黏膜上皮细胞模型;经胃蛋白酶和HCl-H2O低pH以及灭活处理后,嗜酸乳杆菌的黏附能力和对照组差异有显著性。反复冻融后的嗜酸乳杆菌黏附能力与对照组差异无显著性。结论胃蛋白酶和HCl-H2O pH2.0会使嗜酸乳杆菌黏附肠黏膜上皮细胞能力下降,热灭活可使嗜酸乳杆菌的黏附能力提高,反复冻融对嗜酸乳杆菌的黏附能力无明显影响。 |
| 关 键 词： | 嗜酸乳杆菌 肠黏膜上皮细胞 黏附能力 |
| 文章编号： | 1005-376X（2007）02-0145-03 |
| 收稿时间： | 2007-01-26 |
| 修稿时间： | 2007-01-26 |
The study on the adhering capacity to intestinal epithelial cells of chick embryo of Lactobacillus acidophilus |
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| WANG Shu-de,QU Lian-da,WEI Ping. The study on the adhering capacity to intestinal epithelial cells of chick embryo of Lactobacillus acidophilus[J]. Chinese Journal of Microecology, 2007, 19(2): 145-147,149 | |
| Authors: | WANG Shu-de QU Lian-da WEI Ping |
| Affiliation: | 1. The Lab of Microbiology of Animal Medical College of Liaoning Medical University,Jinzhou 121001 ,China;2. The Lab of Preventive Veterinary Medicine of Liaoning Medical University, Jinzhou 121001, China; 3. The Lab of Preventive Veterinary Medcicine of Northeast Agricultural University, Harbin 150030, China |
| Abstract: | Objective To set up the model of intestinal epithelial cells and test the adhering capacity of Lactobacillus acidophilus.Method Pepsin,HCl-H2O pH 2.0,freeze-thaw cycle,inactivation etc.were used to test the adhering capacity of Lactobacillus acidophilus.Results The model of intestinal epithelial cells of chick embryo was set up successfully;After treated by pepsin,HCl-H2O pH 2.0 and inactivation,the adhering capacity of Lactobacillus acidophilus was significantly lower than that of HH control group;The adhering capacity of Lactobacillus acidophilus treated by freeze-thaw cycle didn't change obviously compared to the HH control group.Conclusions Pepsin and HCl-H2O pH 2.0 can make the adhering capacity of Lactobacillus acidophilus decreased;Inactivation by heating can make the adhering capacity of Lactobacillus acidophilus improved;Freezethaw cycle can't influence the adhering capacity of Lactobacillus acidophilus obviously. |
| Keywords: | Lactobacillus acidophilus Intestinal epithelial cells Adhering capacity |
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