Bacterial rhamnolipids are novel MAMPs conferring resistance to Botrytis cinerea in grapevine |
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Authors: | ANNE-LISE VARNIER LISA SANCHEZ PARUL VATSA LESLIE BOUDESOCQUE ANGELA GARCIA-BRUGGER FANJA RABENOELINA ALEXANDER SOROKIN JEAN-HUGUES RENAULT SERGE KAUFFMANN ALAIN PUGIN CHRISTOPHE CLEMENT FABIENNE BAILLIEUL & STEPHAN DOREY |
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Institution: | URVVC-EA 2069, Stress, Défense et Reproduction des Plantes,;UMR CNRS 6229, IFR 53, Institut de Chimie Moléculaire de Reims, Universitéde Reims Champagne-Ardenne, BP 1039, F-51687 Reims cedex 2, France,;UMR Plante-Microbe-Environnement, INRA 1088, CNRS 5184, Universitéde Bourgogne, 17 rue Sully, BP 86510, 21065 Dijon cedex, France,;Institut de Biologie Moléculaire des Plantes du C.N.R.S., UniversitéLouis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg, France and;Algentech Ltd, Institute of Food Research IFR2, Norwich Research Park, Colney Lane, Norwich NR4 7UA, UK |
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Abstract: | Rhamnolipids produced by the bacteria Pseudomonas aeruginosa are known as very efficient biosurfactant molecules. They are used for a wide range of industrial applications, especially in food, cosmetics and pharmaceutical formulations as well as in bioremediation of pollutants. In this paper, the role of rhamnolipids as novel molecules triggering defence responses and protection against the fungus Botrytis cinerea in grapevine is presented. The effect of rhamnolipids was assessed in grapevine using cell suspension cultures and vitro-plantlets. Ca2+ influx, mitogen-activated protein kinase activation and reactive oxygen species production form part of early signalling events leading from perception of rhamnolipids to the induction of plant defences that include expression of a wide range of defence genes and a hypersensitive response (HR)-like response. In addition, rhamnolipids potentiated defence responses induced by the chitosan elicitor and by the culture filtrate of B. cinerea . We also demonstrated that rhamnolipids have direct antifungal properties by inhibiting spore germination and mycelium growth of B. cinerea . Ultimately, rhamnolipids efficiently protected grapevine against the fungus. We propose that rhamnolipids are acting as microbe-associated molecular patterns (MAMPs) in grapevine and that the combination of rhamnolipid effects could participate in grapevine protection against grey mould disease. |
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Keywords: | Vitis vinifera L (grapevine) MAMPs plant defence potentiation |
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