芽孢杆菌SC27胞外代谢产物的活性与成分分析

以从红树林土壤分离并经紫外线和亚硝基胍复合诱变获得的SC27突变菌株作为目标菌,对其胞外代谢产物的活性与成分进行分析。结果表明:芽孢杆菌SC27产生乳酸,产量为5.04 g/L;发酵液蛋白酶、淀粉酶和纤维素酶活力分别为1 316.6、513.3和176.2 U/mL,未检测出脂肪酶;胞外代谢产物对革兰氏阳性菌的抑菌活性强,且抑菌活性物质可耐受高温及木瓜蛋白酶、蛋白酶K和胰蛋白酶处理;发酵液二氯甲烷萃取物的主要化学成分及相对含量为二丁基羟基甲苯(10.28%)、二甲基二氧基硅烷(7.87%)、2,4-二叔丁基苯酚(2.92%)和2个未确定化合物(4.47%、2.36%)。

Analysis of activity and components on extracellular metabolites of Bacillus SC27

The activities and components of extracellular metabolites of a mutant Bacillus SC27 which was isolated from mangrove soil and was mutated via combined nitrosoguanidine (NTG) and UV were analyzed. The results showed that the Bacillus SC27 produced lactic acid of 5.04 g/L. The activities of protease, amylase, cellulase in the fermentation fluid were 1 316.59 U/mL, 176.2 U/mL and 513.3 U/mL, respectively, while no lipase activity was detected. The extracellular metabolic products showed higher anti-bacteria activities against Gram-positive bacteria than those against Gram-negative bacteria, and the anti-bacterial products of Bacillus SC27 could withstand treatment of high temperature, enzymes of papain, proteinase K and trypsin. Main chemical compositions of fermentation fluid extracted by dichloromethane were hydroxytoluene of 10.28%, dimethoxydimethylsilane of 7.87%, 2,4-bis(1,1-dimethylethyl)-phenol of 2.92% and 2 unidentified compounds of 4.47% and 2.36%.