| Abstract: | No comparative study could be found for the analgesic activity of mucuses from the
Oncorhynchus mykiss (OM), Salvelinus fontinalis (SF),
Salmo coruhensis (SC), Acipenser gueldenstaedtii (AG),
and Acipenser baerii (AB) fish species in the literature. We aimed to
investigate the effects of mucuses obtained from the abovementioned fish species on
scalpel incision-induced pain in the rat paw and to examine the role of
oxidant/antioxidant parameters and COX-2 gene expression in the analgesic activities.
Animals were divided into groups: SIC (scalpel incision; SI), SIDS (SI+25 mg/kg diclofenac
sodium), SOM (SI+25 mg/kg OM mucus), SFM (SI+25 mg/kg SF mucus), SCM (SI+25 mg/kg SC
mucus), SAgM (SI+25 mg/kg AG mucus), SAbM (SI+25 mg/kg AB mucus), and HG (healthy). The
paw pain thresholds were measured with a Basile algesimeter before and after diclofenac
sodium (DS) or mucus administration, and then the rats were euthanized with thiopental
sodium. Oxidant/antioxidant and COX-2 gene expression parameters were measured in paw
tissues. OM, SC, AG, and AB fish mucuses could not decrease the SI-induced pain. However,
SF fish mucus prevented this pain by 69% after the first hour and by 58.3% after the third
hour. DS was shown to suppress pain more weakly than SF, preventing the pain by 62.1% and
50.0% after the first and third hours, respectively. SF mucus and DS significantly
inhibited increase of COX-2 gene expression, while other fish mucuses could not. None of
the fish mucuses except SF mucus in conjunction with DS could significantly inhibit the
increase in oxidant parameters and decrease in antioxidants. SF fish mucus should be
comparatively assessed in clinical practice for treatment of postoperative pain. |
