Amino acids metabolism by VO 208 hybridoma cells: some aspects of the culture process and medium composition influence |
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Authors: | A Martial-Gros JL Goergen JM Engasser A Marc |
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Institution: | (1) Laboratoire des Sciences du Génie Chimique – CNRS-ENSAIA, Institut National Polytechnique de Lorraine (INPL), 2, Avenue de la Forêt de Haye, F-54505 Vandoeuvre-lès-Nancy, France |
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Abstract: | In the present study an approach has been developed in order to examine the consequence of essential and non essential amino
acid supplementation on VO208 hybridoma cells behaviour. The effect of amino acid enrichment has been studied taking into
account the culture process, i.e., batch or continuous culture mode and the medium composition, i.e., a home made serum-free
medium or a serum containing one. A group of 4 amino acids, i.e., Ser, Pro, Gly and Arg presented atypical evolution pattern
of their extracellular concentration depending on the type of the medium and on the culture mode. Some amino acids were probably
involved in the limitation of the cellular proliferation. Met was one of the amino acids that appears to may have been at
limiting concentration in all cases. In continuous culture mode, an enrichment of amino acids resulted in a rapid improvement
of the viable cell density in both media, with or without the presence of serum. For most amino acids, supplementation during
continuous culture induced an increase of the amino acid uptake rate. A comparative analysis of amino acids utilisation, depending
on the culture conditions studied in the present study, has been performed in order to propose an overall picture of amino
acids metabolism by VO 208 Hybridoma cell line.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Amino acids Batch culture Continuous culture Hybridoma Kinetics Medium composition Metabolism Serum |
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