An interspecific (Capsicum annuum ×C. chinese) F2 linkage map in pepper using RFLP and AFLP markers |
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| Authors: | B C Kang S H Nahm J H Huh H S Yoo J W Yu M H Lee B-D Kim |
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| Institution: | (1) Department of Horticulture, and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Suwon 441-744, Korea Fax:+82-31-2962768, KR;(2) Jung-Bu Breeding and Research Station, Hung-Nong Seed Co, Eumsung 369-830, Korea, KR |
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| Abstract: | We have constructed a molecular linkage map of pepper (Capsicum spp.) in an interspecific F2 population of 107 plants with 150 RFLP and 430 AFLP markers. The resulting linkage map consists of 11 large (206–60.3 cM)
and 5 small (32.6–10.3 cM) linkage groups covering 1,320 cM with an average map distance between framework markers of 7.5
cM. Most (80%) of the RFLP markers were pepper-derived clones, and these markers were evenly distributed across the genome.
By using 30 primer combinations, we were able to generate 444 AFLP markers in the F2 population. The majority of the AFLP markers clustered in each linkage group, although PstI/MseI markers were more evenly distributed than EcoRI/MseI markers within the linkage groups. Genes for the biosynthesis of carotenoids and capsaicinoids were mapped on our linkage
map. This map will provide the basis of studying secondary metabolites in pepper.
Received: 20 October 1999 / Accepted: 3 July 2000 |
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| Keywords: | Amplified fragment length polymorphism (AFLP) Capsicum Linkage map Restriction fragment length polymorphism (RFLP) |
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