| 硝基还原酶结构域蛋白1在大鼠睾丸组织中特异性高表达在人类睾丸癌中表达下调 |
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| 引用本文： | 王卫,李小玲,李文娟,易梅,唐珂,郑盼,向波,李桂源.硝基还原酶结构域蛋白1在大鼠睾丸组织中特异性高表达在人类睾丸癌中表达下调[J].生物化学与生物物理进展,2013,40(7):652-661. |
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| 作者姓名： | 王卫 李小玲 李文娟 易梅 唐珂 郑盼 向波 李桂源 |
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| 作者单位： | 中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013,中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013,中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013,中南大学湘雅医院皮肤科,长沙 410008,中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013,中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013,中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013,中南大学湘雅医学院肿瘤研究所,长沙 410008;非可控炎症与肿瘤湖南省重点实验室,长沙 410013 |
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| 基金项目： | 国家自然科学基金(81000883, 81272254, 81102065, 91229122),111计划(111-2-12),湖南省科技计划(2011FJ3111),高等学校博士学科点专项科研基金(20110162120009),中南大学自由探索计划(201012200017, 2011QNZT138),中南大学研究生创新基金会(2010ssxt048, CX2011B051, 2012zzts142)资助项目 |
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| 摘 要： | 通过克隆分离鉴定得到大鼠硝基还原酶结构域蛋白1(rNOR1),发现rNOR1 cDNA含有1 418 个碱基,编码含379个氨基酸残基的rNOR1蛋白．rNOR1与人类NOR1 (hNOR1)和小鼠NOR1 (mNOR1)的同源性分别为89% 和93%,这三种同源蛋白都含有OSCP1家族的保守结构域．rNOR1基因在大鼠睾丸中选择性高表达,而且与之同源的人类hNOR1也选择性高表达于睾丸中．通过免疫组化检测人类不同睾丸癌中的hNOR1蛋白表达,发现hNOR1蛋白在非癌变睾丸组织和胚胎性癌组织中高表达,而在精原细胞癌和分化型非精原细胞癌(畸胎瘤,卵黄囊瘤)中低表达．这些数据表明,hNOR1可能是一种睾丸选择性表达基因,睾丸癌hNOR1表达的改变或许可以帮助我们阐明hNOR1蛋白在生殖细胞系肿瘤发生中的功能．
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| 关 键 词： | 硝基还原酶结构域蛋白1(NOR1) 肿瘤发生 睾丸癌 |
| 收稿时间： | 2012/9/16 0:00:00 |
| 修稿时间： | 2012/11/21 0:00:00 |
Testis Selective Expression of NOR1 Gene in Rat and Down Regulation of NOR1 Protein in Human Testicular Germ Cell Tumors |
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| WANG Wei,LI Xiao-Ling,LI Wen-Juan,YI Mei,TANG Ke,ZHENG Pan,XIANG Bo and LI Gui-Yuan.Testis Selective Expression of NOR1 Gene in Rat and Down Regulation of NOR1 Protein in Human Testicular Germ Cell Tumors[J].Progress In Biochemistry and Biophysics,2013,40(7):652-661. |
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| Authors: | WANG Wei LI Xiao-Ling LI Wen-Juan YI Mei TANG Ke ZHENG Pan XIANG Bo and LI Gui-Yuan |
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| Institution: | Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China,Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China,Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China,Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410008, China,Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China,Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China,Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China and Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410008, China;Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China |
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| Abstract: | Rat oxidored nitro domain containing protein 1 (rNOR1) was identified and characterized. Isolated rNOR1 cDNA consisted of 1 418 base pairs that encoded a 379-amino acid protein, and the amino acid sequence was 89% and 93% identical to that of human NOR1 (hNOR1) and mouse NOR1 (mNOR1), respectively. The rat NOR1 protein contained a putative conserved domain belong to OSCP1 superfamily. The message for rNOR1 is highly detected in rat testis. Furthermore, human NOR1 homologue is enriched in testis. By immunostaining human NOR1 protein was detected in human testicular tumors of different subtypes on tissue microarray. NOR1 was strongly immunostained in non-cancerous testicular tissues and embryonal carcinomas, while seminomatous tumour cells and differentiated nonseminomatous derivatives (teratoma, yolk sac tumor) stained less intensely. These data suggested that rNOR1 might serve as a testis-selective gene, and that altered NOR1 expression in testicular cancer may help us to elucidate the functions of NOR1 protein in germ line cells carcinogenesis. |
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| Keywords: | oxidored nitro domain containing protein 1 carcinogenesis testicular cancer |
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