Effects of linker insertions on the biogenesis and functioning of the Escherichia coli outer membrane pore protein PhoE |
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Authors: | Henning Schmidt |
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Institution: | (1) Institut für Genetik, Technische Universität Braunschweig, Spielmannstrasse 8, D-3300 Braunschweig, Federal Republic of Germany |
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Abstract: | Summary The swi1
+ gene is necessary for effective mating-type (MT) switching in Schizosaccharomyces pombe. It was cloned on a 4.2 kb genomic DNA fragment. By site-directed integration into the genome and gene disruption experiments it was proved that the swi1
+ gene itself and not a suppressor had been isolated. Disruption of the swi1
+ gene causes a phenotype identical to that of the original swi1 mutant, i.e. the strain still shows some MT switching. The swi1 gene is unique in the genome and gives rise to a 3 kb mRNA. |
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Keywords: | Mating-type switching Switching genes Double-strand break Schizosaccharomyces pombe |
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