食管癌前细胞DNA、端粒酶含量及多基因表达产物的定量检测

为探讨食管癌高发区人群食管上皮癌变过程中的早期分子改变及早期癌变机理.应用流式细胞术和免疫荧光技术及碘化丙啶DNA荧光染色方法，对食管上皮癌前细胞的DNA含量、端粒酶含量和多个基因p53、p16、cyclin D1蛋白质表达进行了定量检测.检测结果发现，DNA含量在癌变形成时明显增加，异倍体率为87.9%；p53蛋白积聚发生在癌变早期，在癌细胞组的阳性率为100%（5/5）；抑癌基因p16在癌变早期有明显缺失；癌基因cyclin D1及端粒酶阳性率在癌细胞组都为100%（分别为6/6，7/7）.研究结果表明：在癌变早期，DNA含量及异倍体率增加，癌基因cyclin D1表达增高，抑癌基因p16缺失及p53蛋白积聚，端粒酶含量也明显增高，在癌形成时已有多个分子事件发生.

A Quantitative Detection of DNA, Telomerase Content and Multi-gene Expression in Esophageal Precancerous Cell

In order to investigate its alteration in the molecular events and early carcinogenesis mechanism of esophageal epithelium cell in the high incidence area of esophageal cancer, content of DNA,telomerase and multi gene p53,p16,cyclin D1 expression in esophageal precancerous cell were quantitative detected by flow cytometry with indirect immunofluorescence technique and DNA propidium iodide fluorescence staining methods. The detected results showed the DNA content increased singnificantly and the heteroploid rate was 87 9% in occurred carcinogenesis. The p53 protein accumulated and p16 was deleted in the early carcinogenesis of esophagus. The positive rate of p53 was 100%(5/5) in the cancea group. The telomerase and oncogene cyclin D1 were overexpression in the cancer group and their positive rates were 100%(respectively 6/6,7/7), the results indicate that DNA content and heteroploid rate increased, tumor suppressor gene p16 deleted and p53 protein accumulated while telomerase and cyclin D1 protein overexpressed in the early carcinogenesis of esophageal epithelium. There were multiple molecular events occurred when the esophageal carcinoma happened.