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Accelerated polymer-polymer click conjugation by freeze-thaw treatment |
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| Authors: | Takemoto Hiroyasu Miyata Kanjiro Ishii Takehiko Hattori Shota Osawa Shigehito Nishiyama Nobuhiro Kataoka Kazunori |
| Institution: | Department of Materials Engineering and §Department of Bioengineering, Graduate School of Engineering, ?Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, and ∥Center for NanoBio Integration, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. |
| Abstract: | Herein, we report a unique technique to accelerate polymer-SNA conjugation based on copper-free click chemistry: gradual freeze-thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature. |
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