Over-expression of peptide deformylase in chloroplasts confers actinonin resistance,but is not a suitable selective marker system for plastid transformation |
| |
Authors: | Alicia?Fernández-San Millán Patricia?Obregón Email author" target="_blank">Jon?VeramendiEmail author |
| |
Institution: | 1.Instituto de Agrobiotecnología,Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía,Pamplona,Spain |
| |
Abstract: | Arabidopsis thaliana peptide deformylase PDF1B was expressed in tobacco chloroplasts using spectinomycin as the selective agent. The foreign protein
accumulated in chloroplasts (6% of the total soluble protein) and was enzymatically active. Transplastomic plants were evaluated
for resistance to the peptide deformylase inhibitor actinonin. In vitro seed germination in the presence of actinonin and
in planta application of the inhibitor demonstrated the resistance of the transformed plants. In addition, transgenic leaf
explants were able to develop shoots via organogenesis in the presence of actinonin. However, when the combination of the
PDF1B gene and actinonin was used as the primary selective marker system for chloroplast transformation of tobacco, all developed
shoots were escapes. Therefore, under the experimental conditions tested, the use of this system for plastid transformation
would be limited to function as a secondary selective marker. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|