果蝇细胞凋亡核心机制的基因组比较

基因组比较研究是从基因组序列推测调控网络的主要途径。细胞凋亡信号网络是调控网络的一个典型代表。ＥＧＬ１、ＣＥＤ３、ＣＥＤ４和ＣＥＤ９及其同源蛋白质的线虫和哺乳动物构成保守的凋亡核心机制。目前果蝇细胞凋亡核心机制尚不完整,还未找到ＥＧＬ１和ＣＥＤ９类似蛋白质。通过一系列基于生物信息学的基因组比较分析,在果蝇的基因组数据库中发现了两个ＢＣＬ２／ＣＥＤ９和一个ＥＧＬ１的同源蛋白质的编码基因,并重构了果蝇

Conservation and Evolution of the "Core Apoptotic Engine": Lesson from the Ge nome Comparison of Drosophila

Genome comparison is the main approach to deduce regul atory network from genome sequence. Apoptotic network is one kind of typical reg ulatory networks. EGL1, CED3, CED4, CED9 and their homologous proteins play esse ntial roles in apoptosis of C.elegans and mammals, and were regarded as the components of the "core apoptotic engine". But in fruit fly, Drosophila mela nogaster, this network is incomplete. A series of bioinformatic analyses found the lost chains of "core apoptotic engine" by discovering two homologues of B CL2/CED9 and one of EGL1 in fruit fly genome sequences. These findings proved th at the "core apoptotic engine" is indeed widely conserved among multicellular organisms and the evolutionary complexity of this network of Drosophila is b etween that of C.elegans and mammals.