| 热休克后表达受抑基因的分子克隆及其表达特性 |
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| 引用本文： | 戴建新,刘宇建.热休克后表达受抑基因的分子克隆及其表达特性[J].生物化学与生物物理学报,2000,32(6):657-660. |
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| 作者姓名： | 戴建新 刘宇建 |
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| 作者单位： | [1]第二军医大学分子遗传学教研室,上海 [2]第二军医大学病理生理学教研室,上海 |
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| 基金项目： | 国家自然科学基金资助项目，No .3940 0 15 2~~ |
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| 摘 要： | 以人成骨肉瘤细胞株ＨＯＳ－８６０３为热休克模型，在利用ＤＤｍＲＮＡ方法筛选和克隆了一个热休克后表达受抑基因的ｃＤＮＡ片段的基础上，以该片段为探针，筛选ＨＯＳ－８６０３细胞的ｃＤＮＡ文库，获得该基因的全长ｃＤＮＡ克隆（ＨＳＳＧ－１）；ＤＮＡ序列测定表明该ｃＤＮＡ全长１４５６ｂｐ，编码２７６个氨基酸，经计算机辅助分析，该ｃＤＮＡ序列尚未被报道。经ＲＮＡ斑点杂交证实，该基因的表达于多种组织细胞之中，并且
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| 关 键 词： | 热休克 基因表达 表达调控 差别显示 基因克隆 |
Molecular Cloning of a Gene Suppressed by Heat Shock and Its Expression Characte ristics |
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| DAI Jian Xin ,WANG Yi Lun,ZHOU Feng Juan,LU De Ru.Molecular Cloning of a Gene Suppressed by Heat Shock and Its Expression Characte ristics[J].Acta Biochimica et Biophysica Sinica,2000,32(6):657-660. |
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| Authors: | DAI Jian Xin WANG Yi Lun ZHOU Feng Juan LU De Ru |
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| Institution: | DAI Jian Xin *,WANG Yi Lun,ZHOU Feng Juan,LU De Ru |
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| Abstract: | On the base of a cDNA fragment cloned from a human ost eosarcoma cell line (HOS 8603) which was taken as the model of heat shock by DD RT PCR, the cDNA library was screened by the probe made from this fragment, and the colony containing full length cDNA of this cDNA fragment (HSSG 1) was identified. Sequencing data indicated that this cDNA consisted of 1 456 bp, codi ng for 276 amino acids. Computer aided homology analysis did not find the same s equence published. The result of RNA dot hybridization suggested that HSSG 1 was expressing in many kinds of organs and cells, and the down regulating of i ts expression level caused by heat shock was general. Therefore, this may be a g ene that is suppressed by heat shock. |
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| Keywords: | heat shock gene expression expression regulation differential display gene cloning |
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