肌醇四磷酸的制备及其对猪血红蛋白的修饰

经肌醇四磷酸修饰，血红蛋白能改善氧亲和力。小麦麸皮浸出液经硫酸铵分级沉淀、Ｓｅｐｈａｄｅｘ Ｇ－５０凝胶过滤、Ｍｏｎｏ Ｑ离子交换柱梯度层析纯化得到植酸酶。用纯化的植酸酶催化植酸（肌醇六磷酸，ＩＰ６）水解，可得到一系列肌醇磷酸化合物。控制反应条件使水解终止在肌醇四磷酸（ＩＰ４）为主要产物。用国产７１４树脂填充柱一步纯化得到肌醇四磷酸钠盐，经ＨＰＬＣ反向柱，ＦＡＰ质谱分析其中不含其他肌醇磷酸化合物。

Preparation of Inositol Tetrakisphosphate and Its Application in Modification of Porcine Hemoglobin

Modification of hemoglobin using inositol tetrakisphosphate (IP 4) can improve the oxygen affinity of hemoglubin. Phytase was extracted from wheat bran and purified by ammonium sulphate fractionation, followed by Sephadex G 50 gel filtration and Mono Q chromatography. The purified phytase was used for hydrolysis of phytic acid under controled conditions. IP 4, as a major composition of the hydrolysate, was further purified on resin 714 column. The purified IP 4 was oxidized by periodate to obtain dialdehyde IP 4. By the reaction between the aldehyde group of IP 4 derivative and the amino group of porcine hemoglobin (pHb), pHb IP 4 conjugate was formed and was found to have better ability of O 2 binding and releasing than the native hemoglobin.