FEN—1基因反义阻断细胞株（FL—FEN—1^—）的建立

ＦＥＮ－１是一咱结构特异性核酸酶，它在ＤＮＡ复制和修复过程中都起着重要的作用。将ＦＥＮ－１基因的ＮｃｏＩ－ＢａｍＨＩ片段反向克隆到哺乳动物细胞重组表达质粒ｐＭＡＭｎｅｏＡｍｐ＾－中，得到ＦＥＮ－１反义表达质粒ｐＭＡＭｎｅｏＡｍｐ＾－ＦＮＢ＾－，并转染ＦＬ细胞，经Ｇ４１８筛选后，获得ＦＥＮ－１基因表达被阻断的哺乳动物细胞ＦＬ－ＦＥＮ－１＾－。对细胞生长曲线的测定发现，ＦＬ－ＦＥＮ－１＾－在地塞米松的

Establishment of Cell Line with the FEN 1 Gene Blocked by Its Antisense

FEN 1 is a structure specific endo/exonuclease, which is involved in the process of both DNA duplication and DNA repair. In this work a mammalian expression vector expressing antisense FEN 1 gene fragment pMAMneoAmp -FNB - was constructed, after cloning the Nco I Bam HI fragment of FEN 1 gene into the mammalian expression vector pMAMneoAmp - in antisense orientation. After FL cell was transfected with pMAMneoAmp -FNB - and selected by G418, the FL FEN 1 - cell line, in which the FEN 1 gene expression was blocked, was established. It was found that the growth of FL FEN 1 - was decreased upon the induction with dexamethasone and its T D was 3.03 d, while the T D of controls FL and FL M induced with dexamethasone was 2.03 and 2.22 d, respectively, and the T D of the FL FEN 1 - cell without dexamethasone was 2.38 d.