人心肌肌球蛋白轻链1的克隆,表达纯化和单抗制备

报道了中国人心肌肌球蛋白轻链１ｃＤＮＡ的核苷酸序列，并由此推算的氨基酸序列。与国外发表的人心肌肌球蛋白轻链的氨基酸序列比较，发现有两处差异，即在２４位，由谷氨酸变为丙氨酸，则从９８位起至１０１位有４个氨基酸序列的连续差异，即由天冬酰胺－精氨酸－丝氨酸－赖氨酸变为赖氨酸－脯氨酸－精氨酸－谷氨酰妥，推测可能是由于人种差异而引起的。利用该ｃＤＮＡ在大肠杆菌内的表达产物，已获得一株高效的抗中国人心肌肌球蛋

The Sequence Analysis and Expression of cDNA of Human Cardiac Myosin Light Chain 1 and the Preparation of Monoclonal Antibody to the Expressed Product

The nucleotide sequence of cDNA of ventricular myosin light chain 1 of Chinese patients was analyzed. Two remarkable differences in deduced amino acid sequence were found by comparison with amino acid sequence reported previously by Jackowski. The cDNA was expressed in E.coli and the expressed product was used for production of specific polyclonal and monoclonal antibodies. Using both of the poly and monoclonal antibodies, as well as the expressed product, diagnosis kits for acute myocardial infarction was constructed (China patent application number: 98 122066.5). Detailed studies on physiological function of cardiac myosin light chain 1 is under way in our lab.