|
|||||
|
|
| 人内皮生长抑制素基因克隆表达及其抑瘤作用 | |
| 引用本文： | 何志勇 吴祥甫. 人内皮生长抑制素基因克隆表达及其抑瘤作用[J]. Acta biochimica et biophysica Sinica, 2000, 32(4): 333-336 |
| 作者姓名： | 何志勇 吴祥甫 |
| 作者单位： | [1]中国科学院上海生物化学研究所 [2]上海交通大学生命科学技术学院 |
| 摘 要： | 内皮生长抑制素（ｅｎｄｏｓｔａｔｉｎ是最近报道具有抑制肿瘤生长和铁蛋白质，为此，从正常人肝细胞株Ｌ０２中抽提总ＲＮＡ，以ＲＴ－ＰＣＲ法获得了人内皮生长抑制素编码序列，序列分析表明，内皮生长抑制素ＣＤＮＡ开放阅读框架全长５５５ｂｐ，此基因（ＧｅｎＢａｎｋ登录号为Ａ地８４０６０），共编码１、８４个氨基酸残序列为５个碱基与文献报道不同，蛋白质序列中有３个氨基酸残基与文献报道不同，说明存在人种间差异。将人 |
| 关 键 词： | 内皮生长抑制素 抑瘤作用 基因克隆 基因表达 |
Cloning, Expression and Tumor Suppression of Human Endostatin |
|
| HE Zhi Yong ,,CHEN Zhe Yu ,,QIU Cun Ping ,LI Biao ,ZHANG Wei Jie ,WU Xiang Fu. Cloning, Expression and Tumor Suppression of Human Endostatin[J]. Acta biochimica et biophysica Sinica, 2000, 32(4): 333-336 | |
| Authors: | HE Zhi Yong CHEN Zhe Yu QIU Cun Ping LI Biao ZHANG Wei Jie WU Xiang Fu |
| Affiliation: | HE Zhi Yong 1,2,CHEN Zhe Yu 1,3,QIU Cun Ping 1,LI Biao 1,ZHANG Wei Jie 2,WU Xiang Fu 1* |
| Abstract: | Human endostatin cDNA was cloned from total RNA of normal Chinese liver cell line L02 by RT PCR. Endostatin DNA sequence encoded 184 amino acid residues. Five base pairs and 3 amino acid residues are different from that reported, it may be due to interspecies difference. The endostatin cDNA was inserted into the pET 28a( ) containing T7 promoter. The recombinant plasmid was transformed the E.coli BL21(DE3). Recombinant human endostatin was highly expressed as inclusion body when the expression strain BL ENDO was induced with 1 mmol/L IPTG. Result of SDS PAGE analysis revealed that recombinant human endostatin was accounted for up to 25% of soluble protein in E.coli . Purified and refolded recombinant human endostatin was active in inhibiting tumor growth and metastasis. |
| Keywords: | endostatin E.coli cloning expression tumor repression |
| 本文献已被 维普 等数据库收录！ | |