腺苷受体拮抗剂对帕金森病小鼠神经递质的影响

运用神经毒剂ＭＰＴＰ（１－ｍｅｔｈｙ－４－ｐｈｅｎｙ－１，２，３，６－ｔｅｔｒａｈｙｄｒｏｐｙｒｉｄｉｎｅ）制作的ＩＣＲ小鼠帕金森病模型，通过荧光测定方法和免疫组织化学方法，测定ＭＰＴＰ及腺甘Ａ２ａ受体拮抗剂喹唑啉（Ｑｕｉｎａｚｏｌｉｎｅ，ＣＰ６６７１３）对单胺类神经递质去甲肾上腺素（ＮＡ）、多巴胺（ＤＡ）、５－羟色胺（５－ＨＴ）及其代谢产物５－羟吲哚乙酸（５－ＨＩＡＡ）和氨基酸类神经递质γ－氨基

Influence of An Adenosine Receptor Antagonist on Neurotransmitters in Parkinsoni an Mice

The effects of the adenosine A 2a receptor ant agonist, Quinazoline, on the monoamine transmitters NA, DA, 5 HT and 5 HIAA wa s studied using fluorecent method, and the influence of MPTP(1 methyl 4 pheny l 1,2,3,6 tetrahydropyridine) and Quinazoline (CP66713) on the GABA positive r eaction cells in globus pallidus was determined by immunohistochemistry techniqu e in MPTP treated Parkinsonian mice. The results showed that MPTP decreased the content of DA, 5 HIAA and increased 5 HT, GABA obviously(p<0.05, p<0.0 1). Quinazoline functioned opposite the action of MPTP, increasing the content o f DA, 5 HIAA and decreasing the 5 HT. Quinazoline also reduced the GABA posit ive reaction cells in globus pallidus of control mice and maintained the level o f GABA positive reaction cells of globus pallidus in MPTP treated mice, making it similar with the level of control.