Liver glutathione S-transferase polymorphism in Japanese and its pharmacogenetic importance |
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| Authors: | Shoji Harada Masato Abei Naomi Tanaka Dharam P Agarwal H Werner Goedde |
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| Institution: | (1) Institute of Community Medicien, University of Tsukuba, Nihari-Gun, 300-31 Ibaraki-Ken, Japan;(2) Institute of Clinical Medicine, University of Tsukuba, Nihari-Gun, 300-31 Ibaraki-Ken, Japan;(3) Institut für Humangenetik der Universität, Butenfeld 32, D-2000 Hamburg 54, Federal Republic of Germany |
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| Abstract: | Summary A total of 168 autopsy liver extracts from Japanese individuals were examined for the glutathione S-transferase (GST) isozymes by means of starch gel electrophoresis. The gene frequencies of GST1*1, GST1*2, and GST1*0 in Japanese were 0.252, 0.057, and 0.691, respectively. GST1*3 was detected as a rare variant allele. The incidence of GST1 0 in 41 liver biopsy samples from patients suffering from various liver diseases was investigated using polyacrylamide gel isoelectric focusing. The GST1 0 phenotype was found more frequently in livers with hepatitis and carcinoma than in control livers. The isozymes coded by different GST loci were partially purified and characterized to study their biochemical properties. The apparent Km values with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate for the isozymes at the GST1, GST2, GST3, and GST4 loci were 604, 1345, 776 and 591  M, respectively. |
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