Direct shoot formation in spontaneously occurring root pseudonodules of alfalfa (Medicago sativa L.) |
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| Authors: | P Sarul M Vlahova A Ivanova A Atanassov |
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| Institution: | (1) Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria;(2) Institute of Forage Crops, Ulanbatar, Mongolia;(3) Norman Borlaug Centre for Plant Science Research, De Montfort University, 2232 Kostinbrod-2, Bulgaria |
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| Abstract: | Summary A simple and rapid procedure for direct organogenesis from root nodulelike structures of alfalfa (Medicago sativa L.) line SGg, spontaneously induced on growth regulator-free Gamborg (B5) medium, was developed. Prolific adventitious shoot initiation was obtained using a combination of 1.0 mg/liter TIBA and
0.5 mg/liter 2iP. Transfer of shoots to a medium containing 0.5 mg/liter ABA and reduced concentration of TIBA (0.5 mg/liter)
before rooting markedly stimulated shoot development. Regenerated shoots rooted easily and revealed the early appearance of
nodulelike structures on basal medium (B5) lacking growth regulators. Analysis of endogenous growth regulator levels of SGg roots maintained on growth regulators free
media, showed that spontaneous shoot appearances was correlated with high cytokinin-to-auxin ratios. |
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| Keywords: | alfalfa Rhizobium nodulation organogenesis endogenous growth regulators |
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