Novel cell culture device enabling three-dimensional cell growth and improved cell function |
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Authors: | Bokhari Maria Carnachan Ross J Cameron Neil R Przyborski Stefan A |
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Institution: | School of Biological and Biomedical Science, Durham University, South Road, Durham, UK. |
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Abstract: | A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a thin membrane of polystyrene scaffold with a well defined and uniform porous architecture and have adapted this material for cell culture applications. We have exemplified the application of this technology by growing HepG2 liver cells on 2- and 3-D substrates. The performance of HepG2 cells grown on scaffolds was significantly enhanced compared to functional activity of cells grown on 2-D plastic. The incorporation of thin membranes of porous polystyrene to create a novel device has been successfully demonstrated as a new 3-D cell growth technology for routine use in cell culture. |
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Keywords: | Polymer scaffold 3-D cell growth Cell culture HepG2 cells Liver Device |
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