Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese |
| |
| Authors: | Chang Kil Kim Jeung-Sul Han Hyun-Suk Lee Joung-Youl Oh Thoshiro Shigaki Sung Hun Park Kendal Hirschi |
| |
| Institution: | (1) Department of Horticulture, Sangju National University, Sangju, 742-711, Korea;(2) National Horticultural Research Institute, Rural Development Administration, Suwon, 441-440, Korea;(3) Department of Horticultural Research, Gyeongbuk Agricultural Technology Administration, Daegu, 702-708, Korea;(4) Vegetable and Fruit Improvement Center, Texas A&M University, College Station, TX 77845, USA;(5) Baylor College of Medicine, Departments of Pediatrics and Human and Molecular Genetics, USDA/ARS Children’s Nutrition Research Center, 1100 Bates Street, Houston, TX 77030, USA |
| |
| Abstract: | Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B a variant of N-terminus truncated form of CAX2 (sCAX2) containing the “B” domain from CAX1] that has enhanced calcium (Ca2+) substrate specificity and lost the manganese (Mn2+) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50–65% more calcium (Ca2+) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn2+). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca2+ nutrient enrichment of potatoes with reduced accumulation of undesirable metals. |
| |
| Keywords: | Arabidopsis Calcium H+/Ca2+ antiporter Manganese Potato |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
