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Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Cytoplasmic microtubules, vinblastine-induced paracrystals, and mitotic figures.
Authors:K Weber  T Bibring  M Osborn
Affiliation:1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 USA;2. Department of Molecular Biology, Vanderbilt University, Nashville, TN 37240, USA
Abstract:Antibody against tubulin from the outer doublets of sea urchin sperm flagella reacts with tubulin-containing structures in mammalian cells. Thus cytoplasmic microtubules, vinblastine-induced paracrystals and the full spectrum of mitotic figures can be visualized by immunofluorescence. These results show that the tubulin structure has been highly conserved during evolution.
Keywords:Present address: Max Planck Institut für Biophysikalische Chemie   D-34 Göttingen   West Germany   to whom reprint requests should be sent.
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