| 在我国发现普马拉病毒新亚型 |
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| 引用本文： | 唐丽华,张全福,修梅红,扈光伟,沈博,杨显达,梁米芳,李德新.在我国发现普马拉病毒新亚型[J].病毒学报,2007,23(4):320-325. |
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| 作者姓名： | 唐丽华 张全福 修梅红 扈光伟 沈博 杨显达 梁米芳 李德新 |
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| 作者单位： | 传染病预防控制国家重点实验室,中国疾病预防控制中心,病毒病预防控制所,北京,100052;吉林省疾病预防控制中心,长春,130062 |
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| 摘 要： | 对我国东北地区捕获的157份棕背鼠平的肺组织进行普马拉病毒检测.其中免疫荧光检测出阳性标本1份,PCR检测出核苷酸阳性标本7份.对PCR产物进行测序后发现,其核苷酸的序列与报道的普马拉病毒核苷酸序列存在着差异.系统进化分析表明,我国发现的这株普马拉病毒,在进化树上形成了一个新的分支,为一新亚型.并且与在韩国和日本发现的普马拉病毒亲缘关系最为接近.
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| 关 键 词： | 普马拉病毒 S片段 M片段 核苷酸序列分析 |
| 文章编号： | 1000-8721（2007）04-0320-06 |
| 修稿时间： | 2007-05-312007-07-03 |
Identification of a New Puumala Hantavirus Subtype in Rodents from China |
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| TANG Li-hua,ZHANG Quan-fu,XIU Mei-hong,GU Guang-wei,SHEN Bo,YANG Xian-da,LIANG Mi-fang,LI De-xin.Identification of a New Puumala Hantavirus Subtype in Rodents from China[J].Chinese Journal of Virology,2007,23(4):320-325. |
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| Authors: | TANG Li-hua ZHANG Quan-fu XIU Mei-hong GU Guang-wei SHEN Bo YANG Xian-da LIANG Mi-fang LI De-xin |
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| Institution: | 1. State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China ; 2. Center for Disease Control and Prevention of Jilin Province ,Jilin 130062 ,China |
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| Abstract: | In order to detect the nucleic acid of Puumala hantavirus,RNA was extracted from lungs of bank voles captured in Northeast China,and partial S and M genome segments of Puumala virus were amplified by RT-PCR and sequenced.Phylogenetic analysis suggested that Chinese Puumala virus had diverged from the common node of PUUV,with accumulating nucleotide substitutions and formed a distinct lineage from other Puumala viruses.Newly found Puumala virus was most closely related to the Kamiiso-8Cr-95 and Tobetsu-60Cr-93 strains which came from Japan and the muju strains which came from South Korea.By analysis of S and M genome segments of Puumala virus,we deduced a new Puumala virus subtype did exist in Northeast China. |
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| Keywords: | Puumala hantavirus S genome segment M genome segment analysis of nucleotide sequences |
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