异丙酚对人肺血管内皮细胞中ACEⅡ表达的影响

目的:观察异丙酚对人肺动脉内皮细胞中ACE2的影响。方法:以人肺动脉内皮细胞为研究对象,利用Real-time PCR检测不同浓度(1、10、20、40、50μmol/L)异丙酚在不同时间点(6、12、18、24、30h)对HPAEC中ACE2 mRNA表达的影响;Western Blot检测不同浓度(1、10、20、40、50μmol/L)异丙酚对HPAEC中ACE2蛋白表达的影响;观察磷脂酰肌醇-3-激酶(PI3K)抑制剂LY294002对异丙酚调节HPAEC中ACE2 mRNA表达的影响。结果:异丙酚呈剂量和时间依赖性可提高人肺动脉内皮细胞ACE2mRNA水平(P0.05)。但异丙酚浓度为1μmol/L时对ACE2 mRNA水平表达无明显影响(P0.05)。Western Blot检测结果显示异丙酚可增加HPAEC中ACE2蛋白的表达,且在24 h内具有剂量依赖性。异丙酚在24h时剂量依赖性提高Akt的磷酸化,而LY294002可逆转异丙酚对Akt磷酸化的影响。结论:异丙酚可分别通过PI3K/Akt信号途径上调ACE2的表达,使RAS轴处在动态平衡中,从而发挥舒张血管和镇痛作用。

Effect of Propofol on ACE2 Expression of Human Pulmonary Artery Endothelial Cells

Objective： To observe the effect of propofol on Angiotensin-Converting Enzyme 2 （ACE2） expression of human pulmonary artery endothelial cells. Methods： Original generation human pulmonary artery endothelial cells （HPAEC） were taken as the objects. Real-time PCR were used to detect the effect of different concentrations （1, 10, 20, 40,50μmol/L） ofpropofol for different time （6,12,18,24,30h） on theACE2 mRNA expression in HPAEC; Western blot was also applied to detect the ACE2 protein expression in HPAEC after treated by propofol of different concentrations （1,10,20,40,50 mmol/L）. Observe the effect of phosphatidylinositol 3-kinase （PI3K） inhibitor LY294002 on the regulation of propofol. Results： Propofol increased the ACE2 mRNA levels of treated cells in dose- and time-dependent manner （P〈0.05）, but had no effect on the ACE2 mRNA level when with a concentration of 1μmol/L. Western Blot detection results showed that propofol could increase the HPAEC ACE2 protein expression, and in a dose-dependent manner within 24 hours. In 24 hours, propofol increased the Akt phosphorylation dose-dependently, and LY294002 down-regulated the phosphorylation of Akt. Conclusions： Propofol increased the expression of ACE2 through the PI3K/Akt signaling pathways and rebalanced RAS axis, and thus played a role in relaxing blood vessels and had analgesic effects.