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| 被孢霉γ-亚麻酸高产菌株选育 | |
| 引用本文： | 朱国胜,刘作易,雷邦星,廖国会.被孢霉γ-亚麻酸高产菌株选育[J].菌物学报,2005,24(1):85-92. |
| 作者姓名： | 朱国胜 刘作易 雷邦星 廖国会 |
| 作者单位： | 1. 贵州省农业生物技术重点实验室,贵阳,550006;贵州省农业科学院生物技术研究所,贵阳,550006 2. 贵州省农业生物技术重点实验室,贵阳,550006 3. 贵州赤天化股份有限公司科研中心,贵阳,550001 4. 贵州省农业科学院植物保护研究所,贵阳,550006 |
| 基金项目： | 贵州省农业科学院基金(200306) |
| 摘 要： | 利用紫外线复合氯化锂诱变高山被孢霉SM96,筛选出一株高产γ-亚麻酸的菌株TM11,产量比出发菌株(25mg/L)提高了近3倍。对影响其多不饱和脂肪酸产生的因子Mg2+,VB6,营养盐溶液,磷源,碳源,氮源酵母膏进行了正交试验,选出TM11最佳的培养基配方:MgSO4·7H2O1.0g,VB6150mg/L,酵母膏6g/L,葡萄糖100g/L,营养盐溶液1mL/L,K2HPO42g/L。在上述最佳培养基中TM11的生物量达18.0213g/L,总脂达10.8128g/L,GLA量达668mg/L。 |
| 关 键 词： | 微生物油脂 高山被孢霉 诱变 |
| 文章编号： | 1672-6472(2005)01-0085-0092 |
| 修稿时间： | 2004年7月28日 |
SELECTION OF HIGH-YIELDING STRAINS OF MORTIERELLA PRODUCING GAMMA-LINOLENIC ACID BY USE UV AND LiCl |
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| Authors: | ZHU Guo-Sheng LIU Zuo-Yi LEI Bang-Xing LIAO Guo-Hui |
| Institution: | ZHU Guo-Sheng1,2 LIU Zuo-Yi1 LEI Bang-Xing3 LIAO Guo-Hui4 |
| Abstract: | High-yielding strain TM 11 of Mortierella alpina producing gamma-linolenic acid was selected by use of UV and LiCl. The yield of ?-linolenic acid was about 3 times that of its start strain SM96. In this research, some work about selecting optimum fermentation conditions was done. With orthogonal design, the optimum medium formula for the production of ?-linolenic acid was obtained, which contained MgSO47H2O1.0g, VB6 150mg/L, yeast extract 6g/L, glucose100g/L, nutritional salt solution1mL/L, K2HPO42g/L.The TM11 had a biomass of18.0213 g/L and 10.8128 g/L oil and 668mg/LGLA in this medium. |
| Keywords: | Microbial oil Mortierella alpina mutation |
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