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| 绵羊Cytb基因序列多态性及系统进化研究 | |
| 引用本文： | 王昕,马月辉,陈宏. 绵羊Cytb基因序列多态性及系统进化研究[J]. 遗传学报, 2006, 33(12): 1081-1086 |
| 作者姓名： | 王昕 马月辉 陈宏 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,杨凌,712100;中国农业科学院畜牧研究所,北京,100094 2. 中国农业科学院畜牧研究所,北京,100094 3. 西北农林科技大学动物科技学院,杨凌,712100;徐州师范大学生物技术研究所,徐州,221116 |
| 基金项目： | 国家自然科学基金;国家自然科学基金 |
| 摘 要： | 以8个中国地方绵羊品种和1个外来品种的20个个体为研究对象，通过对Cytb基因的全序列测定，结果表明：绵羊的单倍型多样性为97．1％。所有序列的平均碱基组成为27．1％T，28．5％C，31．4％A及13．0％G，G＋C含量为41．5％，核苷酸多样性为0．602％。在所有序列中共检测到43个变异位点，其中包括40处转换和3处颠换。Fu’S中性检验表明差异不显著（0．10〉P〉0．05），说明绵羊群体未发生群体扩张事件。NJ、ME及UPGMA聚类结果均表明，我国绵羊可分为3个单倍型组，这提示我国绵羊有3个母系起源。 |
| 关 键 词： | 绵羊 系统进化 |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2005-11-232006-05-20 |
Analysis of the Genetic Diversity and the Phylogenetic Evolution of Chinese Sheep Based on Cyt b Gene Sequences |
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| WANG Xin,MA Yue-Hui,CHEN Hong. Analysis of the Genetic Diversity and the Phylogenetic Evolution of Chinese Sheep Based on Cyt b Gene Sequences[J]. Journal of Genetics and Genomics, 2006, 33(12): 1081-1086 | |
| Authors: | WANG Xin MA Yue-Hui CHEN Hong |
| Affiliation: | 1. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 3. Institute of Biotechnology, Xuzhou Normal University, Xuzhou 221116, China |
| Abstract: | The complete sequences of Cyt b gene from 20 individuals belonging to eight Chinese indigenous sheep breeds and one foreign breed were studied. The results showed that the hapolotype diversity of Chinese sheep breeds was 97.1%. The mean nucleotide composition of all the sequences was 27.1% T, 28.5% C, 31.4% A, and 13.0% G. The nucleotide diversity was 0.602%.A total of 43 mutation sites were detected, including 40 transitions and 3 transversions. Fu's test of selective neutrality showed that the sheep populations had no population demographic expansion (0.10 ＞ P ＞ 0.05). The different clustering methods, namely neighbor-joining, minimum evolution, and unweighted pair group method with arithmetic means, all showed a similar result, which indicated that Chinese local sheep had three maternal resources. |
| Keywords: | Cytb sheep Cyt b phylogenetic evolution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |