Direct shoot regeneration from immature inflorescence cultures of <Emphasis Type="Italic">Chlorophytum arundinaceum</Emphasis> and <Emphasis Type="Italic">Chlorophytum borivilianum</Emphasis> |
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Authors: | Sanghamitra Samantaray Saroj V Kumar Satyabrata Maiti |
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Institution: | (1) National Research Centre for Medicinal and Aromatic Plants, Boriavi, Anand, Gujarat, 387310, India |
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Abstract: | Direct shoot regeneration was achieved from immature inflorescence explants of Chlorophytum arundinaceum and C. borivilianum on half-strength Murashige & Skoog (MS) medium supplemented with 3.0 mg L−1 BA, 150 mg L−1 Ads, 0.1 mg L−1 NAA and 3% (w/v) sucrose under a 16-h photoperiod. The shoot buds developed within 2–3 weeks of culture. High frequency of
shoot bud regeneration was achieved when cultured on similar medium in subsequent subcultures. The apex portion (Type I) of
the inflorescence produced more shoot buds as compared to the middle ones (type II). More than 75% of the terminal segment
explants produced shoot buds within 4-week of culture. Response of basal portion (Type III) was negative for shoot bud initiation.
Shoots rooted on half-strength basal MS medium supplemented with half-strength MS medium, 0.1 mg L−1 IAA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house and successfully established in the
soil where 90% of the plants survived. This protocol would be useful for commercial micropropagation and genetic improvement
prograrmme. |
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Keywords: | Chlorophytum in vitro immature inflorescence Liliaceae shoot regeneration |
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