| 十四属蓝藻液泡的检查和分离 |
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| 引用本文： | 宋文贞,夏曦中,郭厚良.十四属蓝藻液泡的检查和分离[J].氨基酸和生物资源,2006,28(4):18-20,24. |
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| 作者姓名： | 宋文贞 夏曦中 郭厚良 |
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| 作者单位： | 武汉大学生命科学学院,生物学实验教学中心,武汉,430072 |
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| 基金项目： | 国家自然科学基金资助项目,编号39870083。 |
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| 摘 要： | 采用压片法,渗透冲击法和原生质球法,对14个属的不同蓝藻进行了液泡存在的检查和分离试验,每种蓝藻都检查到液泡,无一例外。其中鱼腥藻、念珠藻、项圈藻、席藻、简孢藻等都能形成液泡化原生质球,原生质球低渗破裂释放液泡,液泡得率极高,不同种的液泡都耐低渗。因此,本研究证明液泡在蓝藻中的存在是普遍的。
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| 关 键 词： | 蓝藻 液泡 |
| 文章编号： | 1006-8376（2006）04-0018-03 |
| 收稿时间： | 09 21 2006 12:00AM |
| 修稿时间： | 2006-09-21 |
Examination and Isolation of Vacuoles from 14 -Genus Cyanobacteria |
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| SONG Wen-zhen,XIA Xi-zhong,GUO Hou-liang.Examination and Isolation of Vacuoles from 14 -Genus Cyanobacteria[J].Amino Acids & Biotic Resources,2006,28(4):18-20,24. |
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| Authors: | SONG Wen-zhen XIA Xi-zhong GUO Hou-liang |
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| Institution: | College of life sciences, Wuhan University, Wuhan 430072, China |
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| Abstract: | The examination and isolation of vacuoles from 14-genus cyanobacteria were carried out.The 14 genera were Anabaena,Nosfoc,Anabaenopsis,Phormidium,Cylindrospermun,Plectonemaboryanum,Scytonema,Spirulina,Oscillatoria,Lyngbya,Gloeocapsa,Syenchococcus,Synechocystis and Microcystis. All the cyanobacteria had been found to have vacuoles.In the genera Anabaena,Noatoc,Anabaenopsis,Phormidium and Cylindrospernum the cells were prepared into the vacuolated spheroplasts,and vacuoles released by hypotonic treatment at a great rate. |
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| Keywords: | Cyanobacteria vacuoles |
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