Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens |
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| Authors: | Javier Rodríguez-Moya Montserrat Argando?a Fernando Iglesias-Guerra Joaquín J Nieto Carmen Vargas |
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| Institution: | aDepartment of Microbiology and Parasitology;bDepartment of Organic and Pharmaceutical Chemistry, Faculty of Pharmacy, University of Seville, Seville, Spain |
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| Abstract: | Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines. |
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