首页 | 本学科首页   官方微博 | 高级检索  
     


MicroRNA-664a-5p promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by directly downregulating HMGA2
Affiliation:1. Department of Orthopaedics, Gongli Hospital of Pudong New Area, Shanghai, China;2. Ningxia Medical University, Yinchuan, China;1. Department of General Practice, The First Hospital of China Medical University, Shenyang, China;2. Department of Nephrology, The First Hospital of China Medical University, Shenyang, China;1. University of Chinese Academy of Sciences, Beijing, 100049, China;2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China;3. Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan;4. State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, 430062, China;1. School of Biological Sciences, Department of Molecular & Biomedical Science, Research Centre for Infectious Diseases, University of Adelaide, Adelaide, 5005, Australia;2. Institute for Glycomics, Griffith University Gold Coast Campus, Queensland, 4222, Australia;1. Department of Medicine and Clinical Science, Division of Cardiology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi, 755-8505, Japan;2. Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan
Abstract:Osteogenic differentiation of human bone marrow–derived mesenchymal stem cells (BMSCs) has been regarded as a central issue in fracture healing. MicroRNAs (miRNAs, miRs) participate in diverse physiological processes such as osteoblastic differentiation of BMSCs. In this study, we found that miR-664a-5p was upregulated during osteogenic differentiation of human BMSCs, and this upregulation positively correlated with the expression of osteogenic genes Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN). Overexpression of miR-664a-5p promoted the osteogenic differentiation of BMSCs, whereas a knockdown of miR-664a-5p suppressed it. Additionally, high-mobility group A2 (HMGA2) mRNA was identified as a direct target of miR-664a-5p that mediates the function of this miRNA. Overexpression of HMGA2 obviously attenuated miR-664a-5p–induced osteogenic differentiation of BMSCs. Thus, the newly identified miR-664a-5p–HMGA2 pathway expands our understanding of the mechanisms underlying the osteogenic differentiation of human BMSCs, may provide deeper insights into the regulation of this differentiation, and can point to new effective methods for treating osteoporosis.
Keywords:miR-664a-5p  HMGA2  Human bone marrow-derived mesenchymal stem cells  Osteogenic differentiation
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号