| 激光复合诱变选育叶酸高产菌 |
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| 引用本文： | 刘艳,薛正莲,胡刘秀,何淑芳.激光复合诱变选育叶酸高产菌[J].激光生物学报,2009,18(1). |
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| 作者姓名： | 刘艳 薛正莲 胡刘秀 何淑芳 |
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| 作者单位： | 1. 安徽工程科技学院生物化学系,安徽,芜湖,241000
2. 上海悦胜芜湖药业有限公司,安徽,芜湖,241000 |
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| 基金项目： | 安徽工程科技学院青年基金，芜湖市科技计划项目 |
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| 摘 要： | 利用高效液相色谱测定发酵液中叶酸含量,比较产朊假丝酵母(Candida utilis)、异常汉逊酵母(ftan-senula anomala)和枯草芽孢杆菌(Bacillus subtilis)产叶酸能力的高低,从而确定生产叶酸的最佳菌种.出发菌株经紫外照射诱变后,再采用激光复合诱变方式进行进一步的筛选,并对其传代稳定性进行研究,以期进一步获得稳产高产叶酸产生菌突变株.结果表明产朊假丝酵母产叶酸量最高.紫外照射3 min得到的Y1.4菌株产叶酸量与原始菌株相比,产量提高了33.8%.激光一紫外复合诱变后筛选出4株产量较高的菌株,其中以Y2.12产量最高.Y2.12产叶酸量与原始菌株相比,提高了65.8%.经传代培养分析,Y2.12诱变株的产量稳定.该结果表明,激光复合诱变是获得高产叶酸的有效途径.
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| 关 键 词： | 激光复合诱变 叶酸 产朊假丝酵母 |
Screening Folic Acid High-yield Strains by Laser Compound Mutagenesis |
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| LIU Yan,XUE Zheng-lian,HU Liu-xiu,HE Shu-fang.Screening Folic Acid High-yield Strains by Laser Compound Mutagenesis[J].ACTA Laser Biology Sinica,2009,18(1). |
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| Authors: | LIU Yan XUE Zheng-lian HU Liu-xiu HE Shu-fang |
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| Institution: | 1.Department of Biochemistry Engineering;Anhui University of Technology and Science;Wuhu 241000;Anhui;China;2.Shanghai Rosant Wuhu Pharmaceutical CO.;LTD;China |
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| Abstract: | The yield of folic acid was tested by HPLC.The optimal folic acid high-yield strain was chosen by comparing the yield of folic acid from Candida utilis,Hansenula anomala and Bacillus subtilis.The folic acid-producing strain was screened by UV and laser compound mutation,and the hereditary stability from generation to generation was studied.The results showed that the optimal folic acid high-yield strain was Candida utilis.The mutant Y1.4 with higher yield of folic acid was obtained after UV induction for 3 ... |
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| Keywords: | laser compound mutagenesis folic acid Candida utilis |
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