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Is protein degradation correlated with either the charge or size of Lemna proteins?
Authors:R B Ferreira  D D Davies
Institution:(1) School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, UK
Abstract:Evidence is presented that the organelles of Lemna minor do not degrade as functional units. The proteins of Lemna show wide differences in their rates of degradation and ribulose bisphosphate carboxylase (EC 4.1.1.39) has a particularly slow rate of degradation. Contrary to some earlier evidence, we found no correlation between the rate of soluble-protein degradation and either charge or size of proteins. We could find no correlation between protein degradation and subunit size in any of the organelles of Lemna.Abbreviations FPLC fast protein liquid chromatography - PAGE polyacrylamide gel electrophoresis - RuBPCase ribulose-1,5-bisphosphate carboxylase - SDS sodium dodecylsulphate - Tris 2-amino-2-(hydroxymethyl)-1,3-propanediol
Keywords:Chloroplast  Lemna (protein degradation)  Mitochondrion  Peroxisome  Protein deg-radation (size/charge)
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