Is protein degradation correlated with either the charge or size of Lemna proteins? |
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Authors: | R B Ferreira D D Davies |
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Institution: | (1) School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, UK |
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Abstract: | Evidence is presented that the organelles of Lemna minor do not degrade as functional units. The proteins of Lemna show wide differences in their rates of degradation and ribulose bisphosphate carboxylase (EC 4.1.1.39) has a particularly slow rate of degradation. Contrary to some earlier evidence, we found no correlation between the rate of soluble-protein degradation and either charge or size of proteins. We could find no correlation between protein degradation and subunit size in any of the organelles of Lemna.Abbreviations FPLC
fast protein liquid chromatography
- PAGE
polyacrylamide gel electrophoresis
- RuBPCase
ribulose-1,5-bisphosphate carboxylase
- SDS
sodium dodecylsulphate
- Tris
2-amino-2-(hydroxymethyl)-1,3-propanediol |
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Keywords: | Chloroplast Lemna (protein degradation) Mitochondrion Peroxisome Protein deg-radation (size/charge) |
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