Structural and immunochemical studies on the lipopolysaccharide of the ‘T-antigen’-containing mutant Proteus mirabilis R14/1959 |
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| Authors: | Beata Bartodziejska Joanna Radziejewska-Lebrecht Maria Lipinska Yuriy A Knirel Leonid O Kononov Anatoly Y Chernyak Hubert Mayer Antoni Rozalski |
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| Institution: | Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, PL 90-237 Lodz, Poland; N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117913, Russian Federation; Max-Planck Institut für Immunbiologie, Stübeweg 51, D-79108 Freiburg i. Br., Germany |
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| Abstract: | Abstract In DOC-PAGE, lipopolysaccharide (LPS) of Proteus mirabilis R14/1959 (Rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. The isolated polysaccharide, called T-antigen because of similarity with the T1 chain of Salmonella friedenau LPS, contained d -glucose, d -galacturonic acid ( d -GalA), and d -GlcNAc in molar ratios 2:1:1 and was structurally different from the O-antigen of the parental S-strain P. mirabilis S1959 but identical to the O-antigen of another S-strain Proteus penneri 42. The importance of a d -GalA( l -Lys)-containing epitope, most likely present in the core region of LPS, and of GalA present in the T-antigen chain in manifesting the serological specificity of P. mirabilis R14/1959 were revealed using rabbit polyclonal homologous and heterologous R- and O-specific antisera and the appropriate antigens, including synthetic antigens which represent partial structures of various Proteus LPS. |
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| Keywords: | Proteus mirabilis R mutants Proteus lipopolysaccharides O-Antigen T-Antigen Synthetic carbohydrate antigens Serological O-specificity |
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