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Storage and survival of bacteria by ultra-freeze
Authors:L. F. GIBSON  J. T. KHOURY
Affiliation:Scottish Crop Research Institute, Invergowrie, Dundee DD2 5 DA, Scotland
Abstract:L yon , G.D., S mith , K.J. & H eilbronn , J. 1986. The calcium requirement of polygalacturonic acid lyases produced by Erwinia spp. Letters in Applied Microbiology , 2 , 127–129.
The optimum calcium concentration for polygalacturonic acid lyase (PL) activity in culture filtrates of Erwinia spp. varied between isolates but was generally between 0.2 and 1 mmol/l. Isozymes of PL have different calcium optima at approximately 0.2, 1, 1.3, 1.6 and 1.8 mmol/l Ca2+.
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