Steroidal saponin production in callus cultures of Solanum aculeatissimum Jacq. |
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| Authors: | T Ikenaga R Handayani T Oyama |
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| Institution: | (1) Faculty of Environmental Sciences, Nagasaki University, 1–14 Bunkyo-machi, Nagasaki 852–8521, Japan e-mail: ikenaga@net.nagasaki-u.ac.jp Fax: +81-958-8432138, JP;(2) Faculty of Pharmaceutical Sciences, Nagasaki University, 1–14 Bunkyo-machi, Nagasaki 852, Japan, JP |
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| Abstract: | Callus was induced from the epicotyl of S. aculeatissimum, and the relation between culture conditions and the production of steroidal saponins in the callus was studied. The results
indicated that the callus produced the steroidal saponins aculeatiside A and B. The highest production of steroidal saponins
occurred at the middle of the log phase. Optimal conditions for the production of steroidal saponins were culturing on MS
basal medium supplemented with the combination of 0.05 ppm or 0.1 ppm NAA and 10 ppm BA, and fructose as a carbon source,
in the dark at 25 °C. Under these optimal conditions, the callus produced 0.8% (per dry weight) steroidal saponins, or 0.32%
aculeatiside A and 0.48% aculeatiside B.
Received: 24 December 1999 / Revision received: 21 February 2000 / Accepted: 16 May 2000 |
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| Keywords: | Solanum aculeatissimum Steroidal saponins Aculeatiside A Aculeatiside B Callus culture |
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