Affinity of integrin alpha 1 beta 1 from liver sinusoidal membranes for type IV collagen. |
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| Authors: | S C Stamatoglou S Bawumia S Johansson E Forsberg R C Hughes |
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| Affiliation: | National Institute for Medical Research, London, UK. |
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| Abstract: | Hepatic sinusoidal membranes isolated from adult rats were extracted with detergent and fractionated on a wheat germ agglutinin affinity column. Bound glycoproteins were eluted with N-acetyl glucosamine and chromatographed on a type IV collagen affinity column. Recovery of the bound fraction by EDTA and analysis by SDS-PAGE revealed two glycoproteins with apparent molecular weights of 180,000 and 117,000. These were identified immunologically by Western blotting as the alpha and beta subunits of integrin alpha 1 beta 1. |
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