Enrichment, isolation, and characterization of 4-chlorophenol-degrading bacterium Rhizobium sp. 4-CP-20 |
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Authors: | Chu-Fang Yang Chi-Mei Lee |
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Institution: | (1) Department of Environmental Engineering, National Chung Hsing University, Taichung, 402, Taiwan |
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Abstract: | The objectives of this research were to monitor the variations of species in mixed cultures during the enrichment period,
isolate species and identify and characterize the pure 4-chlorophenol (4-CP) degrading strains from enriched mixed cultures.
Strain Rhizobium sp. 4-CP-20 was isolated from the acclimated mixed culture. The DGGE result indicated that strain Rhizobium sp. 4-CP-20 was undetectable at the beginning but detectable after 2 weeks of enrichment. The optimum growth temperatures
for Rhizobium sp. 4-CP-20 were both 36°C using 350 mg l−1 glucose or sodium acetate as the substrate. The optimum pH range for degrading 100 mg l−1 4-CP was between 6.89 and 8.20. Strain Rhizobium sp. 4-CP-20 could degrade 4-CP completely within 3.95 days, as the initial 4-CP concentration was 100 mg l−1. If the initial 4-CP concentration was higher than 240 mg l−1, the growth of bacterial cells and the activity of degrading 4-CP were both inhibited. |
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Keywords: | Biodegradation 4-Chlorophenol DGGE Rhizobium sp 4-CP-20 BIOLOG |
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