酵母菌SH2发酵产物增强干扰素抗病毒活性 及其有效成分的初步研究

The fermentation broth of yeast strain SH-2 (FSH-2) which could enhance the biological potency of human interferon alpha (huIFN-α) was detected. Its enhancing ratio was 1.64～6.86 fold. A group of proteins was seperated and purified from the fermentation supernatant by chromatography on Sephadex G-75 and HPLC. Each protein showed the similar band on PAGE and SDS-PAGE. Their molecular weights ranged from 52 to 72 kD. The proteins were stainded with periodic acid Schiff's agent. Lowry's method and sulfuric acid-phenol method were respectively used to determine the contents of proteins and neutral sugars. The results showed the ratio of protein to sugar was 3∶1. These results indicated that the proteins were extracellular glycoproteins (YEGPs) secreted by yeast strain SH-2. YEGPs could significantly enhance the biological potency of huIFN-α. It was veritied by the experiment of cytopathic effect inhibition in Wish cells. The enhancing ratios were 1.6～2.8 fold and 1.4～4.0 fold, respectively. The enhancing ratio of the elution protein of FSH-2 seperated by Sephadex G-75 was 2.01～5.68 fold. YEGPs alone could not enhance the potency of huIFN-α and had no biological activity as IFNs.

Preliminary Study on Fermentation Broth of Yeast Strain SH2 Enhancing Biological Activity of Interferon Alpha and Its Active Principle