Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery |
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| Authors: | Costanza Emanueli Andrew I U Shearn Abas Laftah Francesca Fiorentino Barnaby C Reeves Cristina Beltrami Andrew Mumford Aled Clayton Mark Gurney Saran Shantikumar Gianni D Angelini |
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| Institution: | 1Bristol Heart Institute, University of Bristol, Bristol, United Kingdom;2National Heart and Lung Institute, Imperial College London, London, United Kingdom;3Institute of Cancer & Genetics, University of Cardiff, Cardiff, United Kingdom;Northwestern University, UNITED STATES |
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| Abstract: | IntroductionExosome nanoparticles carry a composite cargo, including microRNAs (miRs). Cultured cardiovascular cells release miR-containing exosomes. The exosomal trafficking of miRNAs from the heart is largely unexplored. Working on clinical samples from coronary-artery by-pass graft (CABG) surgery, we investigated if: 1) exosomes containing cardiac miRs and hence putatively released by cardiac cells increase in the circulation after surgery; 2) circulating exosomes and exosomal cardiac miRs correlate with cardiac troponin (cTn), the current “gold standard” surrogate biomarker of myocardial damage.ConclusionsThe plasma concentrations of exosomes and their cargo of cardiac miRs increased in patients undergoing CABG and were positively correlated with hs-cTnI. These data provide evidence that CABG induces the trafficking of exosomes from the heart to the peripheral circulation. Future studies are necessary to investigate the potential of circulating exosomes as clinical biomarkers in cardiac patients. |
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