| 青阳参的大孢子发生与雌配子体发育 |
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| 引用本文： | 王定康,孙桂芳,翟书华,郭丽红,王斌.青阳参的大孢子发生与雌配子体发育[J].植物研究,2011(3):265-270. |
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| 作者姓名： | 王定康 孙桂芳 翟书华 郭丽红 王斌 |
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| 作者单位： | 昆明学院生物系;昆明医学院临床技能中心; |
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| 基金项目： | 教育部科学技术研究重点项目(209115); 云南省应用基础研究计划项目(2007C262M) |
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| 摘 要： | 利用常规石蜡制片技术、荧光显微技术、光镜细胞化学技术、电子显微镜技术对青阳参大孢子发生、雌配子体形成过程进行了详细观察。结果显示,青阳参为边缘胎座,胚珠倒生、短珠柄,单珠被,薄珠心型,珠心细胞含有大量的淀粉粒、线粒体和内质网等;大孢子孢原细胞起源于下表皮并直接行使大孢子母细胞的功能;合点端的大孢子分裂形成8-核胚囊;蓼型胚囊;成熟胚囊中有大量淀粉粒;珠孔受精;胚乳在早期发育阶段以游离核形式存在,约在16~32核的阶段细胞壁形成,通常情况下胚乳核的分裂比合子的分裂早,成熟胚乳细胞单核、形状不规则,没有胚乳吸器;胚的发育经过原胚、球型胚和心型胚阶段,茄型;成熟的种子具有种毛,位于珠孔端的珠被表皮细胞是种毛长出的区域,种子中含有大量的脂肪。
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| 关 键 词： | 青阳参 大孢子发生 雌配子体 |
Megasporogenesis and Development of Female Gametophyte in Cynanchum otophyllum Schneid |
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| WANG Ding-Kang SUN Gui-Fang ZHAI Shu-Hua GUO Li-Hong WANG Bin.Megasporogenesis and Development of Female Gametophyte in Cynanchum otophyllum Schneid[J].Bulletin of Botanical Research,2011(3):265-270. |
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| Authors: | WANG Ding-Kang SUN Gui-Fang ZHAI Shu-Hua GUO Li-Hong WANG Bin |
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| Institution: | WANG Ding-Kang1 SUN Gui-Fang2 ZHAI Shu-Hua1 GUO Li-Hong1 WANG Bin1(1.Biology Department,Kunming University,Kunming 650214,2.Clinical Skill center,Kunming Medical College,Kunming 650032) |
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| Abstract: | Using traditional paraffin wax section,fluorescence microscopy,cytochemistry and TEM techniques,the megasporogenesis and development of female gametophyte of Cynanchum otophyllum Schneid were observed in detail.The results showed C.otophyllum was marginal placenta;the ovules are anatropous,tenuinucellate and unitegmic;some endoplasm,starch grains and mitochondria exist in nucellus;the archesporial cell is hypodermal in origin and directly gives rise to the megaspore mother cell;the chalazal megaspore functi... |
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| Keywords: | Cynanchum otophyllum Schneid megasporogenesis female gametophyte |
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