|
|||||
|
|
| 稳定表达人ASB12的C2C12细胞系的建立及鉴定 | |
| 引用本文： | 文斗斗,周军媚,赵明一,胡维新,吴秀山,王跃群.稳定表达人ASB12的C2C12细胞系的建立及鉴定[J].生命科学研究,2012,16(4):283-286. |
| 作者姓名： | 文斗斗 周军媚 赵明一 胡维新 吴秀山 王跃群 |
| 作者单位： | 1. 中南大学生物科学与技术学院,中国湖南长沙,410013 2. 湖南师范大学心脏发育研究中心,发育生物学教育部重点实验室,中国湖南长沙410081;南华大学药学与生命科学学院,中国湖南衡阳421001 3. 湖南师范大学心脏发育研究中心,发育生物学教育部重点实验室,中国湖南长沙410081 |
| 基金项目： | 湖南省自然科学基金资助项目(11JJ3028);国家自然科学基金资助项目(81000035;31071999;30871340) |
| 摘 要： | ASB12(homo sapiens ankyrin repeat and SOCS box containing 12)蛋白含有5个ANK(ankyrin repeat sequence)序列和一个保守的SOCS(suppressor of cytokine signaling)盒结构域,是ASBs(human ankyrin repeat andSOCS box containing protein family,ASB family)家族的成员.人类ASB12基因在成体心肌和骨骼肌组织中特异表达,是成肌分化的候选基因.利用阳离子聚合物转染技术将重组表达质粒pCMV-tag2B-ASB12转染小鼠骨骼肌细胞系C2C12细胞,通过G418筛选、免疫荧光检测、RT-PCR分析、Western blotting检测建立了稳定表达ASB12的细胞系C2C12-ASB12,为研究ASB12在骨骼肌发育及其相关功能提供有用的细胞研究模型. |
| 关 键 词： | ASB12 ANK结构域 SOCS box结构域 C2C12稳定细胞系 |
Establishment and Identification of a Stable Human ASB12-Expressed C2C12 Cell Line |
|
| WEN Dou-dou , ZHOU Jun-mei , ZHAO Ming-yi , HU Wei-xin , WU Xiu-shan , WANG Yue-qun.Establishment and Identification of a Stable Human ASB12-Expressed C2C12 Cell Line[J].Life Science Research,2012,16(4):283-286. | |
| Authors: | WEN Dou-dou ZHOU Jun-mei ZHAO Ming-yi HU Wei-xin WU Xiu-shan WANG Yue-qun |
| Institution: | 1.College of Life Sciences and Technology,Central South University,Changsha 410013,Hunan,China;2.The Center for Heart Development,Key Lab of MOE of Develepmental Biology,Hunan Normal University,Changsha 410081,Hunan,China; 3.College of Pharmacy and Life Science,University of South China,Hengyang 421001,Hunan,China) |
| Abstract: | The human ASB12(Homo sapiens ankyrin repeat and SOCS box containing 12) protein contains five ANK(ankyrin repeat sequence) domains and a SOCS(suppressor of cytokine signaling) box domain,be-longing to the ASBs family.It was reported that ASB12 especially expressed in skeletal and cardiac muscles of adult tissues,which suggested that ASB12 closely associated with skeleton muscle development.To con-struct a stable ASB12-expressed C2C12 cell line,the fusion expression plasmid pCMV-tag2B-ASB12,which was identified by enzyme digestion and sequencing analysis,was transfected into C2C12 cell by cationic polymer.After screening culture by G418,the expression of ASB12 was detected by immunofluorescence,RT-PCR and Western-blotting.The C2C12 cell line that expressing ASB12 stably was established successfully,which provide a cell model for studying the molecular function of ASB12 in skeleton muscle development. |
| Keywords: | ASB12 ANK domian SOCS box domain C2C12 stable cell line |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |