Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat |
| |
Authors: | M Tahir A Pavoni G F Tucci T Turchetta D Lafiandra |
| |
Institution: | (1) Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy;(2) National Agriculture Research Center, Islamabad, Pakistan |
| |
Abstract: | A hexaploid wheat landrace collected from the Baluchistan province of Pakistan was found to possess a novel high-molecular-weight glutenin subunit (HMW-GS). The subunit has a very slow electrophoretic mobility as revealed by SDS-PAGE, and its molecular weight is comparable to that of the highest molecular weight glutenin subunit (2.2 encoded in the D-genome) reported so far in hexaploid wheat varieties and landraces of Japanese origin. Evidence obtained from (PCR) gene amplification studies using the primers specific for Glu-1 loci proved that the gene coding for this novel subunit belongs to the Glu-A1 locus located on the long arm of chromosome 1A. Digestion of the amplified gene (PCR product) with restriction enzymes indicated that the novel gene differs from prevailing Glu-A1 alleles (null, 1 and 2*) by an extra DNA fragment of approximately 600 base pairs. The results also indicated that the novel subunit is most probably a derivative of subunit 2* that has very likely incorporated the 600-bp fragment following a process of unequal crossing over. The present findings were further substantiated by reserved phase high performance liquid chromatography (RP-HPLC) analysis. |
| |
Keywords: | Wheat HMW glutenin genes Glu-A1 PCR RP-HPLC |
本文献已被 SpringerLink 等数据库收录! |