湖南烙铁头蛇毒蛋白组分的双向电泳分析

烙铁头蛇是世界上剧毒的蛇种之一,其所携带的毒素能够导致严重的机体损伤。应用蛋白质双向电泳技术,对湖南烙铁头蛇蛇毒蛋白的蛋白质组分进行分析。通过等电聚焦和SDS-PAGE凝胶电泳分析获得完整的烙铁头蛇毒全蛋白质的图谱,经胶体考马斯亮蓝染色后,应用PDQuest软件对蛋白表达谱进行分析。通过等电聚焦和SDS-PAGE凝胶电泳有83个蛋白质组分被检测出来。其中大约90.00%的蛋白质的相对分子质量(Mr)分布在15～45 kDa之间,大约72.29%的蛋白质等电点(pI)在4.0～7.0之间。通过对烙铁头蛇毒的蛋白组学研究,获得其蛇毒蛋白质组分的表征特点,为后续进一步研究各组分的身份和潜在功能奠定基础,既可以提出新的治疗方案又可以为新的药理应用提供宝贵资源。

Component analysis of Protobothrops mucrosquamatusvenom by two-dimensional electrophoresis

Protobothrops mucrosquamatus is one of venomous snakes in the world.Their venoms contain multiple components causing serious injury to human body.A comprehensive approach to separation and identification of the venom protein from Protobothrops mucrosquamatus collected in Hunan province was described.The venom protein components were separated by two-dimensional electrophoresis(isoelectric focusing as first dimension and SDS-PAGE as the second dimension).After coomassie brilliant blue staining,the images of the gels were scanned and analyzed by the software PDQuest.83 different proteins were detected.The relative molecular mass(Mr) of approximately 90% total proteins was between(15-45) kDa.The isoelectric points(pI) of approximately 72.29% of total proteins were between 4.0-7.0.The proteome of protobothrops mucrosquamatus helps the understanding the component of this snake venom and gives a valuable resource for future development of novel human therapeutics and pharmaceutical applications.