单子叶甘露糖结合凝集素的结构及生物活性

单子叶甘露糖结合凝集素(monocot mannose-binding lectin,MBL)是植物凝集素超家族中具有甘露糖及其衍生物结合专一性的糖结合蛋白,对肿瘤细胞、逆转录病毒具有特异而强烈的抑制作用。作为甘露糖翻译器,MBL在糖组学研究中也扮演着重要的角色。迄今为止,对害虫具有良好抗性的转雪花莲、半夏凝集素基因的水稻、小麦、马铃薯、烟草等农作物均已成功问世,可见MBL在医学和农业应用领域都呈现出巨大的开发价值和广阔的应用前景。本文列举了近20年来新发现的几种典型的MBL,并对其分布、分子结构、糖结合专一性、系统进化、生理作用等方面进行了概述。

The Structure and Bioactivity of Monocot Mannose-binding Lectin

The monocot mannose-binding lectin belongs to the superfamily of plant lectins and exhibits exclusively specificity toward mannose and mannose-binding proteins.As structurally and evolutionary related glycoproteins,MBL is of highly similarity and exerts diverse biological roles.It selectively and efficiently inhibits a wide variety of human and animal viruses(including HIV)and suppresses syncytium formation between persistently HIV-I infected cells and uninfected T cells thus deters virus entry.As the translator of mannose,MBL plays an important role in the research of glycomics.Up till now,upcoming the success of transgenic rice,wheat,tomato and tobacco of GNA which shows potential value of exploitation and prospective application in medicine and agriculture.The distribution,binding specificity,biological activity and molecular evolution of MBL are outlined in this paper.