| UV与DES复合诱变选育高产脂肪酶菌株 |
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| 引用本文： | 李忠英,肖杨娟,罗跃中,贺建兵,陈君.UV与DES复合诱变选育高产脂肪酶菌株[J].氨基酸和生物资源,2011,33(3):6-9. |
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| 作者姓名： | 李忠英 肖杨娟 罗跃中 贺建兵 陈君 |
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| 作者单位： | 湖南化工职业技术学院,湖南,株洲,412004;湖南化工职业技术学院,湖南,株洲,412004;湖南农业大学生命科学院,湖南,长沙,410128 |
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| 摘 要： | 以产脂肪酶菌株BaciUus sp CS-4为出发菌株,进行了UV与硫酸二乙酯(DES)复合诱变处理.筛选出一株高酶活的目的菌株,命名为Bacillus spDE-8.其酶活为每毫升14.85U,比出发菌株提高48.2%.传代实验证明,其遗传性能稳定.
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| 关 键 词： | 紫外线 硫酸二乙酯 复合诱变 脂肪酶 |
Screening of Highly Lipase-Producing Strains with Compound Mutagenesis by UV and DES |
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| LI Zhong-ying,XIAO Yang-juan,LUO Yue-zhong,HE Jian-bin,CHEN jun.Screening of Highly Lipase-Producing Strains with Compound Mutagenesis by UV and DES[J].Amino Acids & Biotic Resources,2011,33(3):6-9. |
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| Authors: | LI Zhong-ying XIAO Yang-juan LUO Yue-zhong HE Jian-bin CHEN jun |
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| Institution: | 1 (1.Hunan Chemical Industry Vocation Technology Institute,Zhuzhou 412004;2.Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | With lipase producing strain Bacillus sp CS-4 as the starting strain,the compound mutagenesis was adopted with UV and DES.The strain Bacillus spDE-8 was selected.Its lipase activity was 14.85U·mL-1,which increased 48.2% the original strain.The passage test proved that this strain had stable genetic characteristics. |
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| Keywords: | UV DES compound mutagenesis lipase |
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